Academic Articles & Research Papers

Sustainable clove-derived nitrogen-doped carbon dots with programmable multimodal fluorescence for metal ion and fluoride sensing

The development of sustainable fluorescent nanomaterials from renewable biomass represents a promising strategy for environmentally benign chemical sensing. In this work, nitrogen-doped carbon dots (N-CDs) were synthesized from clove biomas...

Ratiometric near-infrared fluorescence probes for quantitative monitoring of mitochondrial pH dynamics in Drosophila melanogaster and HeLa cells during metformin treatment.

Toll-like receptor 2 coordinates induction of IL-22 in lung macrophages following organic dust-induced inflammation

IntroductionOrganic dust exposures, particularly in occupational settings, induce airway inflammation and contribute to the development and progression of chronic lung disease and impaired tissue regeneration. Interleukin-22 (IL-22) is a pl...

Protocol to induce de novo lipid droplets in Saccharomyces cerevisiae

Summary: The mechanisms underlying lipid droplet (LD) biogenesis in the endoplasmic reticulum are not completely understood. Here, we present a protocol for inducing de novo LDs containing either triacylglycerol (TAG) or sterol esters (SE) ...

Tumor probability mapping of fluorescence intensity during image-guided surgery of head and neck cancer

Abstract Achieving negative surgical margins remains a critical determinant of local recurrence and survival in head and neck cancer (HNC) surgery. Current intraoperative margin assessment techniques, including frozen section analysis, suff...

Fluorescence detection of tramadol in healthy Chinese volunteers by high-performance liquid chromatography and bioequivalence assessment

Xiao Zhou, Ji Liu Department of Anesthesia, Shanghai Pulmonary Hospital, Tongji University School of Medicine, Shanghai, People’s Republic of China Abstract: This study developed a revised high-performance liquid chromatography fluore...

Electrorefining behavior of a multicomponent copper alloy derived from waste printed circuit boards

Efficient recycling of waste electrical and electronic equipment (WEEE) is a major priority for the circular economy and sustainable resource management. This study proposes and investigates an integrated mechanical, pyrometallurgical, and ...

Cold storage of human precision-cut lung slices in TiProtec preserves cellular composition and transcriptional responses and enables on-demand mechanistic studies

Abstract Background Human precision-cut lung slices (hPCLS) are a unique platform for functional, mechanistic, and drug discovery studies in the field of respiratory research. However, tissue availability, generation, and cultivation time r...

Hydrogen gas (H2) delivered by intraperitoneal injection alleviated methionine- and choline-deficient diet-induced metabolic dysfunction–associated steatotic liver disease in mice via inhibiting GSDMD- and GSDME-mediated pyroptosis

BackgroundHydrogen gas (H2), which is the lightest and diffusible gas molecule, has strong abilities to alleviate excessive oxidative stress, inflammation, and apoptosis. Inhalation of H2 is beneficial for preventing the damage of the lung,...

EPIDEMIOLOGICAL ASPECT OF BOVINE VIRAL DIARRHEA VIRUS- II INFECTION IN DAIRY CATTLE

The purpose of this study was to characterize the clinical and virologic features of the mucosal and thrombocytopenic BVDV infection in infected dairy cattle. Strategy of examination included clinical examination of diseased animals, serum ...

A New Strategy for Efficient Screening and Identification of Monoclonal Antibodies against Oncogenic Avian Herpesvirus Utilizing CRISPR/Cas9-Based Gene-Editing Technology

Marek’s disease virus (MDV) is an important oncogenic α-herpesvirus that induces Marek’s disease (MD), characterized by severe immunosuppression and rapid-onset T-cell lymphomas in its natural chicken hosts. Historically, MD is regarded as ...

Characteristics of pulmonary microvascular structure in postnatal yaks

Abstract Yaks are typical plateau-adapted animals, however the microvascular changes and characteristics in their lungs after birth are still unclear. Pulmonary microvasculature characteristics and changes across age groups were analysed us...

Improved Immunohistochemistry of Mouse Eye Sections Using Davidson's Fixative and Melanin Bleaching

Immunohistochemistry (IHC) and immunofluorescence (IF) are fundamental molecular biology techniques to assess protein expression. However, the melanin present normally in the eye in the uveal tract (choroid, iris, and ciliary body) and the ...

Single Eye mRNA-Seq Reveals Normalisation of the Retinal Microglial Transcriptome Following Acute Inflammation

Background: Whether retinal microglia can maintain or restore immune homeostasis during and after inflammation is unclear. We performed single-eye mRNA-sequencing on microglia at different timepoints following a single inflammatory stimulus...

Multi-immersion oblique plane microscope (miOPM): a reconfigurable platform for high-resolution light-sheet fluorescence microscopy.

Handbook of computer vision and applications

Contents of Volume One: Preface. Contributors. B. Jahne, Introduction. Illumination and Image Formation. H. Hausecker, Radiation. H. Hausecker, Interaction of Radiation with Matter. P. Geisler, Geometric and Wave Optics. H. Hausecker, Radio...

Directed Evolution of Near-Infrared Serotonin Nanosensors with Machine Learning-Based Screening

In this study, we employed a novel approach to improve the serotonin-responsive ssDNA-wrapped single-walled carbon nanotube (ssDNA-SWCNT) nanosensors, combining directed evolution and machine learning-based prediction. Our iterative optimiz...

Advancing Biosensors with Machine Learning

Chemometrics play a critical role in biosensors-based detection, analysis, and diagnosis. Nowadays, as a branch of artificial intelligence (AI), machine learning (ML) have achieved impressive advances. However, novel advanced ML methods, es...

Predicting Choroidal Nevus Transformation to Melanoma Using Machine Learning

Purpose: To develop and validate machine learning (ML) models to predict choroidal nevus transformation to melanoma based on multimodal imaging at initial presentation. Design: Retrospective multicenter study. Participants: Patients diagnos...

Prospective evaluation of indocyanine green fluorescence for anastomotic site selection in laparoscopic colorectal cancer surgery.