Optimizing stilbene recovery from cell cultures media: A comprehensive study of the adsorption process

Overview & Synopsis

Stilbenes are of significant interest due to their potential health benefits and applications in pharmaceuticals and nutraceuticals. Traditional extraction methods often involve organic solvents, which pose environmental and safety concerns.This study investigates the extraction of stilbenes (E-resveratrol, labruscol, leachianol, ε-viniferin, and δ-viniferin) from grapevine (Vitis vinifera and Vitis labrusca) cell cultures using adsorption technology. Five food-grade resins (XAD-7, XAD-16, XAD-4, XAD-1180, and FPX-66) were tested for stilbene adsorption. XAD-7 was chosen as the optimum adsorbent, displaying the highest adsorbed quantity (86.94 ± 4.90 mgstilbenes/gdry resin) and desorbed quantity (74.28 ± 0.38 mgstilbenes/gdry resin). Adsorption kinetics using XAD-7 followed a pseudo-second-order model, with intraparticle diffusion limiting approximately 10 % of total adsorption. Desorption occurs more rapidly than adsorption, achieving equilibrium in about 60 min. Isotherm curves fitted well to a multicomponent Langmuir model, indicating a maximum adsorption capacity of 0.280–0.360 mmolstilbenes/gdry resin, close to the experimental value of 0.271 mmolstilbenes/gdry resin. Stilbene affinity for XAD-7 decreased in the following order: ε-viniferin > (labruscol, E-resveratrol, leachianol) > δ-viniferin. The optimal desorbed quantity of 59.74 ± 0.14 mgstilbenes/gdry resin was achieved with a 70 % ethanol solution and a 160:1 desorption solution-to-adsorbent ratio (v/w). XAD-7 resin coupled with an optimized washing step increased stilbene purity by 4.6 times (from 5.41 ± 0.05 % to 23.19 ± 0.31 % w/w). XAD-7 can be reused for multiple cycles with consistent adsorption capacity and desorption yield, maintaining the same stilbenes purity after 5 cycles. This study underscores the viability of polymeric resin adsorption as an eco-friendly and efficient method for stilbene extraction from grapevine cell cultures, paving the way for sustainable production processes in the nutraceutical and pharmaceutical industries.